منابع مشابه
RNA Sequencing Analysis of Salivary Extracellular RNA.
Salivary biomarkers for disease detection, diagnostic and prognostic assessments have become increasingly well established in recent years. In this chapter we explain the current leading technology that has been used to characterize salivary non-coding RNAs (ncRNAs) from the extracellular RNA (exRNA) fraction: HiSeq from Illumina® platform for RNA sequencing. Therefore, the chapter is divided i...
متن کاملDSAP: deep-sequencing small RNA analysis pipeline
DSAP is an automated multiple-task web service designed to provide a total solution to analyzing deep-sequencing small RNA datasets generated by next-generation sequencing technology. DSAP uses a tab-delimited file as an input format, which holds the unique sequence reads (tags) and their corresponding number of copies generated by the Solexa sequencing platform. The input data will go through ...
متن کاملRNA cytosine methylation analysis by bisulfite sequencing
Covalent modifications of nucleic acids play an important role in regulating their functions. Among these modifications, (cytosine-5) DNA methylation is best known for its role in the epigenetic regulation of gene expression. Post-transcriptional RNA modification is a characteristic feature of noncoding RNAs, and has been described for rRNAs, tRNAs and miRNAs. (Cytosine-5) RNA methylation has b...
متن کاملGlobal analysis of RNA cleavage by 5′-hydroxyl RNA sequencing
RNA cleavage by some endoribonucleases and self-cleaving ribozymes produces RNA fragments with 5'-hydroxyl (5'-OH) and 2',3'-cyclic phosphate termini. To identify 5'-OH RNA fragments produced by these cleavage events, we exploited the unique ligation mechanism of Escherichia coli RtcB RNA ligase to attach an oligonucleotide linker to RNAs with 5'-OH termini, followed by steps for library constr...
متن کاملRNA CoMPASS: RNA Comprehensive Multi-Processor Analysis System for Sequencing
Acknowledgments First and foremost, I want to thank my advisor Dr. Christopher Taylor. His endless support and wisdom helped me finish this dissertation. His enthusiasm for Bioinformatics was contagious—and I definitely caught it. His depth of knowledge and very precise academic guidance brought me to develop a web-based GUI distributed computational pipeline, which provides all-in-one function...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2015
ISSN: 1940-3402,1559-6095
DOI: 10.1101/pdb.top084970